Anti-ERP57

Catalog #
AB0004
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB, IHC (F), IHC (P), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal to ERp57 - endoplasmic reticulum lumen marker. This endoplasmic reticulum protein interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. It has disulfide isomerase activity and complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Alternative names
58 kDa glucose regulated protein, 58 kDa microsomal protein, disulfide isomerase ER 60, endoplasmic reticulum resident protein 57, endoplasmic reticulum resident protein 60, ER protein 57, ER protein 60, ER protein 61, ERP57, ERp60, ERp61, glucose regulated protein 58 Kd, GRP57, GRP58, HsT17083, P58, PDIA3, phospholipase C alpha, PI PLC, protein disulfide isomerase A3 antibody.
Gene Identifier/Accession#
Gene ID: 2923, ENSG00000167004
Form
Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Recombinant peptide derived from within residues 300 aa to the C-terminus of human ERp57 produced in E. coli.
Antigen Sequence
STAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRESDFISYLQREATNPPVIQEEKPKKKKKAQEDL
Specificity
Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000
IF 1:50-1:500
IHC (P) 1:200-1:1,000
IHC (F) 1:200-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ +++ ND
Rat +++ +++ +++ +++ ND
Mouse +++ +++ +++ +++ ND
Canine +++ +++ +++ +++ ND
Monkey +++ +++ +++ +++ ND

External References (1)

Images

Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/1,000 dilution, CANX - AB0041 at 1/5,000 and GAPDH - AB0049 at 1/2,500; lysates at 50 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-ERP57 Ab in primary RPE cells at 1/100 dilution; cells were fixed with 4% of PFA;
IHC of human pancreas using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 Ab at 1:750/DAB detection;
IHC of human gallbladder using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 Ab at 1:750/DAB detection;
Immunofluorescence – anti-ERP57 Ab in primary RPE cells at 1/100 dilution; cells were fixed with 4% of PFA;
IHC of mouse kidney using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 Ab at 1:750/DAB detection
Endogenous ERP57- endoplasmic reticulum lumen marker detected at 1/500 dilution; lysates at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
IHC of human appendix using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 Ab at 1:750/DAB detection;
IHC of mouse retina using anti-ERp57 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-ERp57 Ab at 1:750/DAB detection;

300 €

Quantity / Unit Size

600 µg
1.5 mg
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