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Catalog #
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AB0005
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Category
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Antibodies
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Family
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Organelle Markers
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Source
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Goat
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Application
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WB, IF
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Reactivity
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Human, Rat, Mouse, Monkey, Canine
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Description
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Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
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Alternative names
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Early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.
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Gene Identifier/Accession#
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Gene ID: 8411, ENSG00000102189
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Form
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Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant peptide within residues 1,230 aa to the C-terminus of human EEA1 produced in E. coli.
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Antigen Sequence
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KKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
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Specificity
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Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:250-1:1,000
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IF
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1:50-1:250
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Human
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+++
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ND
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ND
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+++
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ND
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Rat
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+++
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ND
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ND
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+++
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ND
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Mouse
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+++
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ND
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ND
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+++
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ND
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Canine
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+++
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ND
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ND
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+++
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ND
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Monkey
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+++
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ND
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ND
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+++
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ND
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Immunostaining of Hepa1-6 cells with EEA1 antibody at 1/50 dilution;
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Endogenous EEA1 detected with Ab at 1/500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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