Anti-EEA1
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Catalog #
AB0005
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB, IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
Alternative names
Early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.
Gene Identifier/Accession#
Gene ID: 8411, ENSG00000102189
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide within residues 1,230 aa to the C-terminus of human EEA1 produced in E. coli.
Antigen Sequence
KKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
Specificity
Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:250-1:1,000
IF 1:50-1:250

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND +++ ND
Rat +++ ND ND +++ ND
Mouse +++ ND ND +++ ND
Canine +++ ND ND +++ ND
Monkey +++ ND ND +++ ND

Images

Immunostaining of Hepa1-6 cells with EEA1 antibody at 1/50 dilution;
Endogenous EEA1 detected with Ab at 1/500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

600 µg
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