Anti-EEA1

Catalog #
AB0006
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB, IHC (F), IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
Alternative names
early endosome antigen 1,162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.
Gene Identifier/Accession#
Gene ID: 8411, ENSG00000102189
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide within residues 1230 aa to the C-terminus of human EEA1 produced in E. coli.
Antigen Sequence
KKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
Specificity
Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:250-1:1,000
IF 1:25-1:250
IHC (F) 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND +++ +++ ND
Rat +++ ND +++ +++ ND
Mouse +++ ND +++ +++ ND
Canine +++ ND +++ +++ ND
Monkey +++ ND +++ +++ ND

Images

Immunostaining of primary fibroblasts with EEA1 antibody at 1/50 dilution;
Anti-EEA1 Ab at 1/500 dilution; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

600 µg
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