Anti-Rab5a
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Catalog #
AB0010
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB, IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to mouse Rab5a. Rab5a belongs to the small GTPase superfamily, Rab family. Rab5a is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
Alternative names
RAB5A, RAS-associated protein RAB5A, ras-related protein Rab-5A antibody.
Gene Identifier/Accession#
ENSG00000144566, Gene ID: 5868
Form
Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a produced in E. coli.
Antigen Sequence
ELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN
Specificity
Detects Rab5a protein by Western blot in cell lysates and transfected cells with GFP-Rab5a. This Ab is specific for Rab5a; it does not recognize Rab5b and Rab5c.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:250-1:2,000
IF 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND +++ ND
Rat +++ ND ND +++ ND
Mouse +++ ND ND +++ ND
Canine +++ ND ND +++ ND
Monkey +++ ND ND +++ ND

Images

Anti-Rab5a Ab at 1/500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Immunofluorescence – anti-Rab5a Ab using NIH3T3 cells transduced with GFP-Rab5a; cells were fixed with methanol and anti-Rab5a at 1/100;
Immunofluorescence – anti-Rab5a Ab using NIH3T3 cells transduced with GFP-Rab5a; cells were fixed with methanol and anti-Rab5a at 1/100;

320 €

Quantity / Unit Size

800 µg
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