Anti-Rab14
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Catalog #
AB0013
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB, IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
RAB14 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking. This protein is expressed at high levels in kidney, lung, brain, spleen and thymus and it is thought to be involved in vesicular trafficking and neurotransmitter release. It is also involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
Alternative names
F protein-binding protein 1; bA165P4.3 (member RAS oncogene family); ras-related protein Rab-14; small GTP binding protein Rab14 antibody.
Gene Identifier/Accession#
ENSG00000119396, Gene ID: 51552
Form
​Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab14 produced in E. coli.
Antigen Sequence
MTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC
Specificity
Detects Rab14 by Western blot in transfected cells with GFP-Rab14.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:250-1:2,000
IF 1:25-1:200

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND +++ ND
Rat +++ ND ND +++ ND
Mouse +++ ND ND +++ ND
Canine +++ ND ND +++ ND
Monkey +++ ND ND +++ ND

Images

Anti-Rab14 Ab at 1/500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

300 €

Quantity / Unit Size

400 µg
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