Anti-Rab2b
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Catalog #
AB0021
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
RAB2 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking. It has been demonstrated that Rab2 is essential for the maturation of pre-Golgi intermediates. RAB2A and RAB2B are required for protein transport from the ER to the Golgi, RAB2A is lipid-anchored to the ER-Golgi intermediate compartment membrane while RAB2B is lipid anchored to the cytoplasmic side of the cell membrane.
Alternative names
Ras-related protein Rab-2A, Ras-related protein Rab-2B antibody.
Gene Identifier/Accession#
ENSG00000129472, Gene ID: 84932
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of Rab2b produced in E. coli.
Antigen Sequence
MGNKSDLESRRDVKREEGEAFAREHGLIFMETSAKTACNVEEAYINTAKEIYRKIQQGLFDVHNEANGIKIGPQQSITSSVGPCSPQQNVSDIGPDSGCC
Specificity
Detects levels of Rab2b protein in transfected cells with GFP-Rab2b by Western blot. This antibody is specific for Rab2b and it barely detects Rab2a.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:250-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-Rab2b Ab at 1/1,000 dilution; HEK293 cells transfected with GFP-Rab2a and GFP-Rab2b; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

600 µg
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