Anti-RPS6

Catalog #
AB0052
Category
Antibodies
Family
Organelle Markers
Source
Goat
Application
WB, IF
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal to RPS6. Ribosomal protein S6 is a component of the 40S ribosomal subunit and is therefore involved in regulating translation – ribosome marker. Studies have also shown to be involved in cell proliferation, regulation of cell size and glucose homeostasis. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases.
Alternative names
RPS6 ribosomal protein S6, S6, 40S ribosomal protein S6, phosphoprotein NP33 antibody.
Gene Identifier/Accession#
ENSG00000137154, Gene ID: 6194
Form
Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Recombinant peptide derived from within residues 190 aa to the C-terminus of human RPS6 produced in E.
Antigen Sequence
MALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK
Specificity
Detects a band of approximately 30 kDa by Western blot in the following whole cell lysates: MDCK, AtT20, Jurkat and 3T3.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IF 1:25-1:250

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND +++ ND
Rat +++ ND ND +++ ND
Mouse +++ ND ND +++ ND
Canine +++ ND ND +++ ND
Monkey +++ ND ND +++ ND

Images

Anti-RPS6 Ab at 1/500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Confocal immunofluorescence of Hepa1-6 cells with RPS6 Ab at 1/100 dilution; methanol fixation;

300 €

Quantity / Unit Size

800 µg
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