Anti-Rab27b

(0) Reviews

(0) References

(0) Validations

(0) Q&A

Catalog #: AB0072
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IHC (F), IHC (P), IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to Rab27b. Rab27b belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in vesicle transport and small GTPase mediated signal transduction.
Alternative names: C25KG, ras-related protein Rab-27B.
Gene Identifier/Accession#: ENSG00000041353, Gene ID: 5874
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27b produced in E. coli.
Antigen Sequence: MFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLIGNKADLPDQREVNERQARELADKYGIPYFETSAATGQNVEKAVETLLDLIMKRMEQCVEKTQIPDTVNGGNSGNLDGEKPPEKKCIC
Specificity: Detects Rab27b by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27b. This antibody is specific for Rab27b. It does not recognize Rab27a protein.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:250-1:2,000
IF	1:50-1:250
IHC (P)	1:100-1:400
IHC (F)	1:100-1:400

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

Images

	Anti-Rab27b Ab at 1/500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Anti-Rab27b antibody IHC staining of mammary tissue; immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval; antibody concentration 1/200;