Anti-ATG12

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Catalog #: AB0083
Category: Antibodies
Family: Organelle Markers
Source: Goat
Application: WB, IHC (F), IHC (P), IF
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Alternative names: APG12, Apg12 (autophagy, yeast) homolog, APG12L, FBR93, HAPG12, autophagy protein 12, autophagy-related protein 12 antibody.
Gene Identifier/Accession#: Gene ID: 9140, ENSG00000145782
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.
Antigen Sequence: AEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGD
Specificity: Detects GFP-ATG12 in transfected cells by Western blot.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:250-1:2,000
IF	1:50-1:250
IHC (F)	1:200-1:1,000
IHC (P)	1:200-1:1,000

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (1)

Kharaziha P, Panaretakis T. Methods Enzymol. 2017;587:247-255.

PMID: 28253959

Images

	Immunofluorescence – anti-ATG12 Ab – Autophagosome Marker in Hepa1-6 cells at 1/50 dilution; cells were fixed with methanol;
	Anti-Atg12 Ab at 1/500 dilution; 293HEK cells transfected with GFP-Atg12; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	IHC of mouse stomach anti-Atg12 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Atg12 Ab at 1:500/DAB detection.
	IHC of mouse kidney using anti-Atg12 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Atg12 Ab at 1:500/DAB detection.
	IHC of mouse liver using anti-Atg12 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Atg12 Ab at 1:500/DAB detection.
	IHC of human pancreas anti-Atg12 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Atg12 Ab at 1:500/DAB detection.
	IHC of human skin anti-Atg12 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Atg12 Ab at 1:500/DAB detection.