Anti-DYKDDDDK
- Catalog #
- AB0085
- Category
- Antibodies
- Family
- TAG-Antibodies
- Source
- Goat
- Application
- WB, IF
- Reactivity
- Transfected cells
- Description
- Goat polyclonal antibody to DYKDDDDK epitope tag.
- Alternative names
- D-tag, ECS Epitope Tag, ECS-tag, FLAG-tag antibody.
- Gene Identifier/Accession#
- N/A
- Form
- Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 3.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Purified recombinant peptide DYKDDDDK produced in E. coli.
- Antigen Sequence
- MDYKDDDDKNPMDYKDDDDKNPMDYKDDDDKNPMDYKDDDDKNPGRLERPHRD
- Specificity
- This antibody recognizes recombinant proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins in transfected cells.
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use.
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Application Values WB 1:500-1:2,000 IF 1:100-1:500 -
Sample WB IHC (F) IHC (P) IF ELISA Transfected cells +++ ND ND ND ND -
Anti-DYKDDDDK Ab at 1/1,000 dilution; 293 hTERT RPE-1 cells transduced with Flag-CAS9 lentivirus; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Usage
Reactivity Chart
External References (1)
Images
320 €
Quantity / Unit Size
600 µg
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