Anti-DYKDDDDK

Catalog #
AB0085
Category
Antibodies
Family
TAG-Antibodies
Source
Goat
Application
WB, IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to DYKDDDDK epitope tag.
Alternative names
D-tag, ECS Epitope Tag, ECS-tag, FLAG-tag antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide DYKDDDDK produced in E. coli.
Antigen Sequence
MDYKDDDDKNPMDYKDDDDKNPMDYKDDDDKNPMDYKDDDDKNPGRLERPHRD
Specificity
This antibody recognizes recombinant proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins in transfected cells.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IF 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-DYKDDDDK Ab at 1/1,000 dilution; 293 hTERT RPE-1 cells transduced with Flag-CAS9 lentivirus; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

600 µg
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