Anti-V5

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Catalog #: AB0096
Category: Antibodies
Family: TAG-Antibodies
Source: Goat
Application: WB, IF
Reactivity: Transfected cells
Description: Goat polyclonal antibody to V5 epitope. V5 peptide corresponds to amino acid residues GKPIPNPLLGLDST (95-108 aa) of the P and V proteins of the Paramyxovirus of simian virus SV5.
Alternative names: GKPIPNPLLGLDST epitope, V5 tag antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide produced in E. coli.
Antigen Sequence: MGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPMGKPIPNPLLGLDSTNPGRLERPHRD
Specificity: Reacts specifically with V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:500-1:5,000
IF	1:50-1:2,000

Reactivity Chart

Sample	WB	IHC (F)	IF	ELISA
Transfected cells	+++	ND	+++	ND

External References (2)

Calado J, Santos AR, Aires I, et al. FEBS Lett 2018 Aug.	PMID: 30156268
Catarino S, Ribeiro-Rodrigues TM, Sa Ferreira R, et al. Cells 2020 Apr.	PMID: 32272685

Images

	Anti-V5 Ab at 1/1,000 dilution; MBP-V5 recombinant protein and 293 cells transfected with V5-mCherry; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed and stained with anti-V5 Ab (1:1,000); donkey anti-goat Alexa 568 labelled secondary Ab was used;
	Immunofluorescence – anti-V5 Ab using hCEC cells transduced with V5-mCherry-Rab11a; cells were fixed with methanol and anti-V5 at 1/100;