Anti-CD45
- Catalog #
- AB0100
- Category
- Antibodies
- Family
- CD Markers
- Source
- Goat
- Application
- WB, IHC (F), IHC (P)
- Reactivity
- Human, Rat, Mouse, Monkey, Canine
- Description
- Goat polyclonal antibody to CD45. It is a 180-220 kDa type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase (PTP) family. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It is expressed in all hematopoietic cells, but highest on lymphocytes. PTPs are known to be signalling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. It has intrinsic tyrosine phosphatase activity and it is essential for T and B cell antigen receptor-mediated activation. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signalling. CD45 also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signalling. The cross-linking of CD45 induces apoptosis in lymphocytes.
- Alternative names
- B220, CD45 antigen, CD45R, GP180, L-CA, LCA, leukocyte common antigen, LY5, protein tyrosine phosphatase, PTPRC, receptor type c polypeptide, receptor-type tyrosine-protein phosphatase C, RP11-553K8.4, T200, T200 glycoprotein, T200 leukocyte common antigen antibody.
- Gene Identifier/Accession#
- Gene ID: 5788, ENSG00000081237
- Form
- Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
- Concentration
- 3.00 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- Unconjugated
- Immunogen
- Purified recombinant peptide derived from within residues 1,260 aa to the C-terminus of human CD45 produced in E. coli.
- Antigen Sequence
- ANCVNPLGAPEKLPEAKEQAEGSEPTSGTEGPEHSVNGPASPALNQGS
- Specificity
- Using Jurkat cell lysate detects a band 180-220 kDa by Western blot.
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Special instructions
- The antibody solution should be gently mixed before use.
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Application Values WB 1:500-1:2,000 IHC (F) 1:250-1:1,000 IHC (P) 1:250-1:1,000 -
Sample WB IHC (F) IHC (P) IF ELISA Human +++ +++ +++ ND ND Rat +++ +++ +++ ND ND Mouse +++ +++ +++ ND ND Canine +++ +++ +++ ND ND Monkey +++ +++ +++ ND ND -
IHC of human appendix using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection. 
Endogenous CD45 detected with at 1/500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution. 
IHC of mouse eye using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:750/DAB detection. 
IHC of human lymph node using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection. 
IHC of mouse liver using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection. 
IHC of rat spleen using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.
Usage
Reactivity Chart
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300 €
Quantity / Unit Size
600 µg
1.5 mg
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