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Catalog #
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AB0126
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Category
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Antibodies
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Family
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TAG-Antibodies
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Source
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Goat
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Application
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WB
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Reactivity
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Transfected cells
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Description
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Goat polyclonal antibody to T7 epitope tag. T7 epitope tag is useful for the labelling and detection of proteins by immunoprecipitation, immunostaining and immunoblotting techniques. Because of its small size, it is unlikely to affect the tagged protein's biochemical properties.
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Alternative names
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MASMTGGQQMG Tag antibody.
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant peptide MASMTGGQQMG produced in E. coli.
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Antigen Sequence
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MASMTGGQQMGNPMASMTGGQQMG
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Specificity
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The antibody recognizes the T7-tag fused to the amino- or carboxy- termini of targeted proteins in transfected cells.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use.
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Application
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Values
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WB
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1:500-1:5,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Transfected cells
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+++
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ND
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ND
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ND
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ND
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Anti-T7 tag Ab at 2,500 dilution; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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