Anti-GroEL

Catalog #
AB0130
Category
Antibodies
Family
Bacteria
Source
Goat
Application
WB
Reactivity
Bacteria
Description
Goat polyclonal antibody to GroEL. GroEL protein is a 60 kDa chaperone that promotes refolding of misfolded polypeptides especially under stressful conditions.
Alternative names
chaperonin GroEL protein antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 400 aa to the C-terminus of Burkholderia GroEL produced in E. coli.
Antigen Sequence
DALHATRAAVEEGIVAGGGVALIRARTAIAGLTGANADQNAGIKIVLRAMEEPLRQIVTNGGEEASVVVAAVAAGKGNYGYNAATGEYVD
Specificity
The Ab recognizes the endogenous protein in Burkholderia and Pseudomonas by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Bacteria +++ ND ND ND ND

Images

Anti-GroEL Ab at 1/2,500 dilution; 50-100 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

600 µg
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