Anti-Luciferase
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Catalog #
AB0131
Category
Antibodies
Family
Fusion / Marker Proteins
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Alternative names
Luc and Luciferin 4-monooxygenase antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
Antigen Sequence
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-Luciferase Ab at 1/500 dilution; 293HEK transduced with lentivirus expressing luciferase; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

300 €

Quantity / Unit Size

600 µg
1.5 mg
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