Anti-Rab35
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Catalog #
AB0198
Category
Antibodies
Family
Vesicle Trafficking
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
RAB35 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking. Rab35 is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic-recycling pathway.
Alternative names
RAB1C, GTP-binding protein RAY, H-ray, ras-related protein Rab-35; ras-related protein rab-1C and RAY antibody.
Gene Identifier/Accession#
ENSG00000111737, Gene ID: 11021
Form
Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab35 produced in E. coli.
Antigen Sequence
MNQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC
Specificity
Detects Rab35 in cell lysates and transfected cells by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:250-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-Rab35 Ab at 1/1,000 dilution; 293HEK transfected with GFP-Rab35; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

800 µg
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