Anti-ALIX
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Catalog #
AB0327
Category
Antibodies
Family
Apoptosis
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to ALIX. Programmed cell death 6 interacting protein (ALIX) functions within the ESCRT pathway in cytokinesis, apoptosis and maintenance of tight junction integrity, membrane repair, intralumenal endosomal vesicle formation and budding of virus.
Alternative names
AIP1, DRIP4, HP95, PDCD6IP, programmed cell death 6 interacting protein antibody.
Gene Identifier/Accession#
Gene ID: 10015, ENSG00000170248
Form
Polyclonal antibody supplied as a 100 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Peptide derived from within residues 840 aa to the C-terminus of human ALIX.
Antigen Sequence
SAPSIPTPAYQSLPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ
Specificity
Detects endogenous levels of ALIX in HUH, MDCK, AtT20, MCF7, COS7, HT1080 and MCR by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Anti-ALIX Ab at 1/1,000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

200 µg
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