Anti-Miranda

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Catalog #: AB0344
Category: Antibodies
Family: Insect
Source: Goat
Application: WB, IF
Reactivity: Fruit fly
Description: Goat polyclonal antibody to Miranda. This protein plays a role in neurogenesis in Drosophila embryos.
Alternative names: CG12249, Dmel\CG12249, mir, Mir, MIR, Mira, MIRA antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Recombinant peptide derived from within residues 220 aa to the N-terminus of Drosophila melanogaster Miranda produced in E. coli.
Antigen Sequence: MSFSKAKLKRFNDVDVAICGSPAASNSSAGSAGSATPTASSAAAAPPTVQPERKEQIEKFFKDAVRFASSSKEAKEFAIPKEDKKSKGLR LFRTPSLPQRLRFRPTPSHTDTATGSGSGASTAASTPLHSAATTPVKEAKSASRLKGKEALQYEIRHKNELIESQLSQLDVLRRHVDQLK EAEAKLREEHELATSKTDRLIEALTSENLSHKALNE
Specificity: Detects endogenous levels of Miranda protein by immunofluorescence.
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:500-1:2,000
IF	1:50-1:500

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Fruit fly	+++	ND	ND	+++	ND

Images

	Immunofluorescence – anti-Miranda Ab in fly cells at 1/100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA;
	Anti-Miranda Ab at 1/1,000 dilution; protein at 40 ng per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-Miranda Ab in fly cells at 1/100 dilution; InsGAL4 UASCD8 GFP wandering L3; cells were fixed with 4% of PFA;