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Catalog #
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AB0348
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Category
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Antibodies
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Family
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Malaria
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Source
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Goat
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Application
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WB
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Reactivity
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Plasmodium
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Description
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HSP20, a heat shock protein, that plays a role in in cell traction and Plasmodium motility.
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Alternative names
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Small heat shock protein antibody.
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Affinity purified recombinant fusion protein HSP20 from Plasmodium and produced in E. coli.
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Antigen Sequence
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MSCLCAGCGDKGEIKITPDHRINIKEKQSVPKNKNTLVNPNTVLEIMPDKNLKKQITFRPKVDIMYDSESCHAILVLDIPGFKIDDIDVEIGEGMLTIAGPRSQTELFETYGDNLVLHAKEREVGYFKRIFKLPHNILDDTAHAVYKNGILEIKMECKQFSYMHKVEINEA
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Specificity
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In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:2,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Plasmodium
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+++
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ND
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ND
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ND
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ND
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ND
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Anti-HSP20 Ab at 1:2,000 dilution; 30 ng of protein per lane; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;
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