Anti-CD81
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Catalog #
AB0361
Category
Antibodies
Family
CD Markers
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. This protein is a cell surface glycoprotein, known to complex with integrins and appears to promote muscle cell fusion and support myotube maintenance, acting as platform for receptor clustering and signalling motility.
Alternative names
CD81 antigen, CVID6, S5.7, TAPA1, Tetraspanin-28, TSPAN28 antibody.
Gene Identifier/Accession#
ENSG00000110651, Gene ID: 975.
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 115 aa to 205 aa of human CD81 produced in E. coli.
Antigen Sequence
NKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLYLIG
Specificity
Reacts with CD81 detected by Western blot using the following human (Jurkat and MCF7), mouse NIH3T3 whole cell lysates.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Human +++ ND ND ND ND ND
Rat +++ ND ND ND ND ND
Mouse +++ ND ND ND ND ND
Canine +++ ND ND ND ND ND
Monkey +++ ND ND ND ND ND

Images

Anti-CD81 Ab at 1/1,000 dilution; lanes with 50 µg of total lysates; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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