Anti-Spike RBD Domain (SARS-CoV-2)
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Catalog #
AB0370
Category
Antibodies
Family
SARS-CoV-2 Virus
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
The spike or S glycoprotein is a transmembrane protein with a molecular weight of about 150 kDa found in the outer portion of the SARS-CoV-2 virus. This protein is composed of two subunits, S1 and S2 and plays a key role in the receptor recognition and cell membrane fusion process. The S1 subunit contains a receptor-binding domain that recognizes and binds to the host receptor angiotensin-converting enzyme 2, while the S2 subunit mediates viral cell membrane fusion.
Alternative names
S glycoprotein SARS Coronavirus-2, Spike RBD Domain SARS Coronavirus-2 antibody.
Gene Identifier/Accession#
N/A
Form
​Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fusion protein using the spike protein RBD domain (residues 320 to 420) and produced in E. coli.
Antigen Sequence
NATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
Specificity
In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-Spike Ab at 1/2,500 dilution; lane with 30 ng of recombinant fusion protein; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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