Anti-NSP1 (SARS-CoV-2)
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Catalog #
AB0385
Category
Antibodies
Family
SARS-CoV-2 Virus
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Host translation inhibitor Nsp1 is part of the multifunctional protein replicase polyprotein 1ab that is involved in the transcription and replication of viral RNA. NSP1 inhibits the host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Therefore, Nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response by suppressing host gene expression.
Alternative names
Host translation inhibitor, Nsp1 SARS Coronavirus-2 antibody.
Gene Identifier/Accession#
N/A
Form
​Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fusion protein Nsp1 of SARS-CoV-2 produced in E. coli.
Antigen Sequence
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG
Specificity
In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-NSP1 Ab at 1/2,500 dilution; lane with 30 ng of recombinant fusion protein; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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