Anti-NSP4 (SARS-CoV-2)
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Catalog #
AB0389
Category
Antibodies
Family
SARS-CoV-2 Virus
Source
Goat
Application
None
Reactivity
Transfected cells
Description
Nsp4 is part of the multifunctional protein replicase polyprotein 1ab that is involved in the transcription and replication of viral RNA. This non-structural protein in cooperation with Nsp3 and Nsp6 has been implicated in rearrangement of host membrane that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication.
Alternative names
Nsp4 SARS Coronavirus-2 antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fusion protein using the C-terminal of Nsp4 (residues 385 to stop) and produced in E. coli.
Antigen Sequence
MVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQ
Specificity
In lysates of transfected cells with the plasmid containing the sequence used, detects the fusion protein by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-NSP4 protein Ab at 1/2,500 dilution; lane with 30 ng of recombinant fusion protein (385 aa - stop); rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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