Anti-mKate
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Catalog #
AB0412
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mKate (Red fluorescent protein). mKate is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mKate is a ~26 kDa protein that is optimally excited at a 588 nm and has a maximum of emission at 635 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
Alternative names
far-Red antibody.
Gene Identifier/Accession#
N/A
Form
​Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND ND ND

Images

Anti-mKate Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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