Anti-FUS
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Catalog #
AB0452
Category
Antibodies
Family
Epigenetics and Nuclear Signaling
Source
Goat
Application
WB
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to FUS. FUS (Fused in Sarcoma), also known as TLS (Translocated in Liposarcoma), is a multifunctional RNA-binding protein encoded by the FUS gene. It is a member of the FET protein family, which also includes EWSR1 and TAF15. FUS plays a critical role in various cellular processes, including transcription regulation, RNA splicing, RNA transport, and DNA repair.
Alternative names
ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS antibody.
Gene Identifier/Accession#
Gene ID: 2521, ENSG00000089280
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human FUS produced in E. coli.
Antigen Sequence
MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGY
Specificity
Detects endogenous levels of FUS by Western blot in the whole cell lysates (H69, HeLa, MCF7, AtT20, LS174T, 293HEK,IMCD3, Jurkat, COS7, SHSY5Y, NIH3T3, etc.).
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:1,000-1:5,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Human +++ ND ND ND ND ND
Mouse +++ ND ND ND ND ND
Rat +++ ND ND ND ND ND
Monkey +++ ND ND ND ND ND
Canine ND ND ND ND ND

Images

Endogenous FUS detected with at 1:2,500 dilution; lysate at 50 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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