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Catalog #
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AB0453
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Category
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Antibodies
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Family
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TAG-Antibodies
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Source
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Goat
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Application
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None
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Reactivity
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Saccharomyces cerevisiae
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Description
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Goat polyclonal antibody to GAL4. The GAL4 reporter system is a widely used genetic tool in molecular biology and developmental biology, primarily in Drosophila melanogaster (fruit fly) research but also adapted for other model organisms like mice and zebrafish. The GAL4 "Driver" Line Encodes the GAL4 transcription factor, originally derived from Saccharomyces cerevisiae (yeast). It is a binary expression system that allows precise spatial and temporal control of gene expression.
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Alternative names
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N/A
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant peptide derived from within residues 140 aa to the N-terminus of yeast Gal4 produced in E. coli.
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Antigen Sequence
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MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKG
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Specificity
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Detects levels of Gal4 recombinant protein by Western blot.
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Saccharomyces cerevisiae
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+++
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ND
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ND
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ND
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ND
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ND
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Gal4 recombinant protein at 40 ng per lane detected with at 1:1,000 dilution and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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