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Catalog #
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AB081550
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Category
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Antibodies
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Family
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Fluorescent Proteins
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Source
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Goat
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Application
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WB, IHC (F), IF
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Reactivity
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Transfected cells
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Description
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Goat polyclonal antibody to RFP (Red fluorescent protein) conjugated to DyLight® 550. RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.
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Alternative names
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Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
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Concentration
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2.50 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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DyLight®550
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Immunogen
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Purified recombinant RFP peptide produced in E. coli.
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Antigen Sequence
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MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
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Specificity
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In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
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Storage
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Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
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Special instructions
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Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:500-1:5,000
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IHC (F)
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1:50-1:1,000
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IF
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1:50-1:500
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Transfected cells
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+++
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+++
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ND
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+++
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ND
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ND
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Immunofluorescence – anti-RFP Ab conjugated to DyLight® 550 using COS-7 cells transduced with GFP-Rab5a (signal amplification); cells were fixed with methanol and anti-RFP at 1/250;
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Anti-RFP Ab conjugated to DyLight® 550 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane;
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