Anti-RFP, DyLight®680
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Catalog #
AB081680
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to RFP (Red fluorescent protein) conjugated to DyLight® 680. RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.
Alternative names
Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Dylight®680
Immunogen
Purified recombinant RFP peptide produced in E. coli.
Antigen Sequence
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:1,000
IF 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ ND +++ ND ND

Images

Anti-RFP Ab conjugated to DyLight® 680 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane;

350 €

Quantity / Unit Size

250 µg
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