Anti-CD45, DyLight®800
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Catalog #
AB100800
Category
Antibodies
Family
CD Markers
Source
Goat
Application
None
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to CD45 conjugated to DyLight®800. It is a 180-220 kDa type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase (PTP) family. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It is expressed in all hematopoietic cells, but highest on lymphocytes. PTPs are known to be signalling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. It has intrinsic tyrosine phosphatase activity and it is essential for T and B cell antigen receptor-mediated activation. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signalling. CD45 also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signalling. The cross-linking of CD45 induces apoptosis in lymphocytes.
Alternative names
B220, CD45 antigen, CD45R, GP180, L-CA, LCA, leukocyte common antigen, LY5, protein tyrosine phosphatase, PTPRC, receptor type c polypeptide, receptor-type tyrosine-protein phosphatase C, RP11-553K8.4, T200, T200 glycoprotein, T200 leukocyte common antigen antibody.
Gene Identifier/Accession#
Gene ID: 5788, ENSG00000081237
Form
Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Dylight®800
Immunogen
Purified recombinant peptide derived from within residues 1,260 aa to the C-terminus of human CD45 produced in E. coli.
Antigen Sequence
ANCVNPLGAPEKLPEAKEQAEGSEPTSGTEGPEHSVNGPASPALNQGS
Specificity
Using Jurkat cell lysate detects a band 180-220 kDa by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ ND ND ND ND
Rat +++ ND ND ND ND
Mouse +++ ND ND ND ND
Canine +++ ND ND ND ND
Monkey +++ ND ND ND ND

Images

Endogenous CD45 detected with Ab conjugated to DyLight® 800 at 1/2,500 dilution; lysate at 50 µg per lane.

350 €

Quantity / Unit Size

250 µg
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