Anti-Rab5 Pan

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Catalog #: AB1024
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IHC (F), IHC (P), IF, ELISA
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to mouse Rab5. Rab5 belongs to the small GTPase superfamily, Rab family. Rab5a is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
Alternative names: RAB5A, RAB5B, RAB5C, RAS-associated protein RAB5A, ras-related protein Rab-5B, RAS-associated protein RAB5C, ras-related protein Rab-5A, ras-related protein Rab-5B, ras-related protein Rab-5C antibody.
Gene Identifier/Accession#: ENSG00000144566
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a, Rab5b and rab5c produced in E. coli.
Antigen Sequence: N/A
Specificity: Detects total Rab5 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab5a, GFP-Rab5b and GFP-Rab5c cds by Western blot.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:250-1:2,000
IHC (F)	1:200-1:1,000
IHC (P)	1:200-1:1,000
IF	1:50-1:250
ELISA	ND

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (2)

Toneatti R, Shin JM, Shah UH, et al. Sci Signal. 2020 Oct.	PMID: 33082287
Burrinha T, Gomes R, Terrasso AP, et al. bioRxiv Apr 2019

Images

	Immunofluorescence – anti-Rab5 Ab using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA;
	IHC of human gallbladder using anti-Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5 Ab at 1:100/DAB detection.
	Anti-Rab5 Ab at 1/1,000 dilution; tissue lysate at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
	IHC of mouse stomach using anti-Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5 Ab at 1:500/DAB detection.
	Immunofluorescence – anti-Rab5 Ab using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA;
	IHC of human myocardium using anti-Rab5 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab5 Ab at 1:100/DAB detection.
	Immunofluorescence – anti-Rab5 Ab using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA;