Anti-CD19
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Catalog #
AB1097
Category
Antibodies
Family
CD Markers
Source
Goat
Application
WB, IHC (F), IHC (P)
Reactivity
Human, Rat, Mouse, Monkey, Canine
Description
Goat polyclonal antibody to CD19. CD19 is a type I transmembrane glycoprotein with 2 C-type Ig domains and multiple phosphorylation sites on long cytoplasmic domain. It is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. It is a major B lineage marker.
Alternative names
B4, Bgp95, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CVID3, differentiation antigen CD19, T-cell surface antigen Leu-12 antibody.
Gene Identifier/Accession#
Gene ID: 930, ENSG00000177455
Form
​Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS. This Ab does not contain preservatives. Glycerol and azide FREE. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant peptide within residues 510 aa to the C-terminus of human CD19 produced in E. coli.
Antigen Sequence
YAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR
Specificity
Using spleen and Jurkat cell lysates detects a 95 kDa band by Western blot. This Ab is similar to AB0097.
Storage
For extended storage, store in -20 C freezer.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IHC (F) 1:250-1:1,000
IHC (P) 1:250-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Human +++ +++ +++ ND ND
Rat +++ +++ +++ ND ND
Mouse +++ +++ +++ ND ND
Canine +++ +++ +++ ND ND
Monkey +++ +++ +++ ND ND

Images

Endogenous CD19 detected with anti-CD19 Ab at 1/500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution. AB1097 Ab is similar to AB0097.

375 €

Quantity / Unit Size

600 µg
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