Anti-Goat IgG, HRP
- Catalog #
- AB1125
- Category
- Antibodies
- Family
- Secondary Antibodies
- Source
- Chicken
- Application
- WB, ELISA
- Reactivity
- Goat
- Description
- Chicken polyclonal antibody to goat IgG conjugated to horseradish peroxidase (HRP).
- Alternative names
- Immunoglobulin Y antibody.
- Gene Identifier/Accession#
- N/A
- Form
- Polyclonal antibody supplied as a 500 µl (2 mg/ml) aliquot in PBS, 50% glycerol and 0.05% sodium azide.
- Concentration
- 2.00 mg/ml
- Isotype
- IgY
- Clonality
- Polyclonal
- Conjugation
- HRP
- Immunogen
- Full goat IgG affinity purified from domestic goat (Capra hircus) serum using protein G. Purity >95% by SDS-PAGE.
- Antigen Sequence
- N/A
- Specificity
- Using human samples detects human IgG by Western blot. Reduced cross-reactivity to other species.
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Special instructions
- The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.
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Application Values WB 1:2,000-1:10,000 ELISA 1:20,000-1:100,000 -
Sample WB IHC (F) IHC (P) IF ELISA Goat +++ ND ND ND +++ -
Western blot using using lysates at 30 µg per lane; 1st Ab anti-GAPDH Ab (AB0049) at 1:2,500 and 2nd Ab anti-goat conjugated to HRP (AB1125) at 1:10,000; 
Anti-HSP20 Ab at 1:2,000 dilution; 30 ng of protein per lane; chicken polyclonal to goat IgG conjugated to HRP (AB1125) at 1/10,000 dilution;
Usage
Reactivity Chart
Images
200 €
Quantity / Unit Size
1 mg
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