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Catalog #
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AB1147
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Category
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Antibodies
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Family
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Secondary Antibodies
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Source
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Goat
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Application
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WB, ELISA
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Reactivity
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Human
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Description
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Goat polyclonal antibody to human IgG. IgG is a monomeric immunoglobulin, with two heavy chains and two light chains. IgG can be found in blood and is the most abundant immunoglobulin, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses (IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%)) that can bind to many types of pathogens, protecting the body against them by complement activation, opsonization for phagocytosis and neutralisation of their toxins.
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Alternative names
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Immunoglobulin G antibody.
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 500 µl (2 mg/ml) aliquot in PBS, 50% glycerol and 0.05% sodium azide.
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Concentration
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2.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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HRP
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Immunogen
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Full human IgG affinity purified from serum of four normal donors tested and found negative for Hepatitis B, Hepatitis C, HIV I and II antibodies using protein G. Purity >95% by SDS-PAGE.
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Antigen Sequence
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N/A
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Specificity
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Using serum samples detects human IgG by Western blot. Reduced cross-reactivity to other species.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles.
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Application
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Values
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WB
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1:2,000-1:10,000
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ELISA
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1:20,000-1:100,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Human
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+++
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ND
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ND
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ND
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+++
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