Anti-HA
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Catalog #
AB129168
Category
Antibodies
Family
TAG-Antibodies
Source
Goat
Application
WB, IF
Reactivity
Transfected cells
Description
G oat polyclonal antibody to HA epitope. The YPYDVPDYA epitope is used as a tag on many recombinant proteins to facilitate isolation, purification and detection of recombinant proteins. The HA tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors.
Alternative names
Human influenza hemagglutinin tag, YPYDVPDYA tag antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
4.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Purified recombinant HA epitope peptide derived from the human Influenza Hemagglutinin protein and produced in E. coli.
Antigen Sequence
MAYPYDVPDYANPMAYPYDVPDYANPMAYPYDVPDYA
Specificity
The antibody recognizes the HA-tag fused to the amino- or carboxy- termini of targeted proteins in transfected cells.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:2,000
IF 1:100-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA
Transfected cells +++ ND ND +++ ND

Images

Anti-HA Ab at 1/1,000 dilution; transfected 293 cells; lysate at 40 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

800 µg
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