Anti-tdTomato, DyLight®650
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Catalog #
AB181650
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to tdTomato (red fluorescent protein) conjugated to DyLight® 650. tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
Alternative names
Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
DyLight®650
Immunogen
Purified recombinant peptide produced in E. coli.
Antigen Sequence
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:2,000
IHC (F) 1:50-1:1,000
IF 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ ND +++ ND ND

Images

Anti-tdTomato Ab conjugated to DyLight® 650 at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane;

350 €

Quantity / Unit Size

250 µg
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