Anti-Venus

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(5) References

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Catalog #: AB2166
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IHC (F), IHC (P), IF, IEM
Reactivity: Transfected cells
Description: Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.
Alternative names: GFP, Green fluorescent protein antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant GFP peptide produced in E. coli.
Antigen Sequence: N/A
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Transfected cells	+++	+++	+++	+++	ND

External References (5)

Zhang H, Xiong X, Guo K, et al. Nat Commun 2024 Jul	PMID: 38956103
Tankmar MD, Reichel M, Arribas-Hernández L, et al. EMBO Rep 2023 Nov	PMID: 38009565
Sarmah P, Shang W, Origi A, et al. Cell Rep 2023 Mar
Tankmar, M;Reichel, M;Arribas-Hernández, L, et al. bioRix 2023
Arribas-Hernández L, Bressendorff S, Hansen MH, et al. Plant Cell. 2018 May	PMID: 29643069

Images

	Immunofluorescence – anti-Venus Ab in 293HEK cells transfected with Venus protein at 1/50 dilution; cells were fixed with 4% of PFA;
	Immunogold labeling of epithelium cells, in vivo injected with Venus expressing vector;
	Anti-Venus Ab at 1/2,000 dilution; 293HEK cells transduced with Venus-Rab7 Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;