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Catalog #
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AB236237
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Category
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Antibodies
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Family
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Metabolism
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Source
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Goat
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Application
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WB
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Reactivity
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Human, Rat
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Description
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Goat polyclonal to C-Peptide. C-Peptide is part of proinsulin, that consists of C-Peptide, alpha and beta chains that later become linked together to form the insulin molecule. Each proinsulin molecule produces one molecule of insulin and one molecule of C-peptide that is released into the blood. C-peptide stimulates the N+, K+ -ATPase and endothelial nitric oxide synthase activities by binding to cell membranes which leads to increased intracellular Ca2+ concentration.
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Alternative names
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N/A
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Gene Identifier/Accession#
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Gene ID: 3630, ENSG00000254647
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Form
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Polyclonal antibody supplied as a 200 µl (4 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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4.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Purified recombinant human and rat C-peptides produced in E. coli as a fusion protein.
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Antigen Sequence
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EAEDLQVGQVELGGGPGAGSLQPLALEGSLQGTEVEDPQVAQLELGGGPGAGDLQTLALEVARQ
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Specificity
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Detects C-peptide using MBP-C-peptide recombinant fusion protein and GFP-C-peptide expressed in transfected mammalian cells.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use.
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Application
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Values
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WB
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1:500-1:2,000
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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Human
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+++
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ND
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ND
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ND
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ND
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Rat
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+++
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ND
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ND
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ND
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ND
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Anti-C-peptide Ab at 1/1,000 dilution; 20 ng of recombinant protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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