Anti-mCardinal
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Catalog #
AB2491
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IHC (P), IF, IEM
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mCardinal (Far Red fluorescent protein). mCardinal is a basic (constitutively fluorescent) far red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity. mCardinal is a ~27 kDa protein that is optimally excited at a 604 nm and has a maximum of emission at 659 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
Alternative names
far-Red antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
IEM 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ ND ND ND ND +++

Images

Anti-mCardinal Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 40 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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