Anti-Mouse IgG, DyLight®550
- Catalog #
- AB27550
- Category
- Antibodies
- Family
- Secondary Antibodies
- Source
- Goat
- Application
- WB, IF
- Reactivity
- Mouse
- Description
- Goat polyclonal antibody to mouse IgG conjugated to DyLight®550.
- Alternative names
- Immunoglobulin G antibody.
- Gene Identifier/Accession#
- N/A
- Form
- Polyclonal antibody supplied as a 200 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
- Concentration
- 2.50 mg/ml
- Isotype
- IgG
- Clonality
- Polyclonal
- Conjugation
- DyLight®550
- Immunogen
- Full mouse IgG affinity purified from mouse (M. musculus) serum using protein G. Purity >95% by SDS-PAGE.
- Antigen Sequence
- N/A
- Specificity
- Using serum samples detects mouse IgG by Western blot. Reduced cross-reactivity to other species.
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Special instructions
- Avoid freeze/thaw cycles.
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Application Values WB 1:2,000-1:10,000 IF 1:100-1:1,000 -
Sample WB IHC (F) IHC (P) IF ELISA IEM Mouse +++ ND ND +++ ND ND -
Immunostaining - C57BL/6J mouse retina labeled with: Green- 1st Ab Cone Arrestin (1/1,000); 2nd Ab goat anti-rat (AB28488, 1/1,000), Red- 1st Ab Rhodopsin (1/1,000); 2nd Ab goat anti-mouse (AB27550, 1/1,000), Blue- Nuclear staining (DAPI), scale bar = 40 um; 
Immunofluorescence using hCEC cells, 1st Ab (anti-beta-Actin at 1/250) and 2nd Ab (anti-mouse AB27550 at 1/1,000); cells were fixed with methanol;
Usage
Reactivity Chart
Images
200 €
Quantity / Unit Size
500 µg
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