Anti-Chicken IgY, DyLight®405

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Catalog #: AB307405
Category: Antibodies
Family: Secondary Antibodies
Source: Goat
Application: WB, IF
Reactivity: Chicken
Description: Goat polyclonal antibody to chicken IgY conjugated to DyLight®405.
Alternative names: Immunoglobulin G antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 200 µl (2.5 mg/ml) aliquot in PBS, 20% glycerol, 0.05% ProClin® and 0.05% sodium azide.
Concentration: 2.50 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: DyLight®405
Immunogen: Full chicken IgY affinity purified from domestic chicken (G. gallus) egg yolk. Purity >95% by SDS-PAGE.
Antigen Sequence: N/A
Specificity: Using serum samples detects chicken IgY by Western blot. Reduced cross-reactivity to other species.
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:2,000-1:10,000
IF	1:100-1:1,000

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA	IEM
Chicken	+++	ND	ND	+++	ND	ND

Images

	Immunofluorescence – anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goat anti-IgY (AB307405) at 1:1,000;
	Immunofluorescence – anti-mCherry Ab (AB9770) using hCEC cells transduced with mCherry-Rab5a; cells were fixed with methanol and mCherry Ab at 1/250; 2nd Ab goat anti-IgY (AB307405) at 1:1,000;