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Catalog #
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AB3336
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Category
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Antibodies
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Family
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Fluorescent Proteins
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Source
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Goat
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Application
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WB, IHC (F), IHC (P), IF, IEM
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Reactivity
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Transfected cells
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Description
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Goat polyclonal antibody to TurboRFP (orange fluorescent protein). TurboRFP is a basic (constitutively fluorescent) orange fluorescent protein, monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. It has very low acid sensitivity. TurboRFP is a ~26 kDa protein that is optimally excited at a 553 nm and has a maximum of emission at 574 nm. This bright fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
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Alternative names
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RFP antibody.
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Gene Identifier/Accession#
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N/A
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Form
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Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
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Concentration
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3.00 mg/ml
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Isotype
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IgG
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Clonality
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Polyclonal
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Conjugation
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Unconjugated
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Immunogen
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Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
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Antigen Sequence
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MASSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKLGHR
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Specificity
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In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
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Storage
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For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
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Special instructions
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The antibody solution should be gently mixed before use.
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Application
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Values
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WB
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1:500-1:5,000
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IHC (F)
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1:50-1:500
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IHC (P)
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1:50-1:500
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IF
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1:50-1:500
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IEM
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1:50-1:500
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Sample
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WB
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IHC (F)
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IHC (P)
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IF
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ELISA
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IEM
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Transfected cells
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+++
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+++
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+++
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+++
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ND
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+++
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Anti-TurboRFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
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