Anti-mRuby
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Catalog #
AB3410
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IHC (P), IF, IEM
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mRuby (Red fluorescent protein). mRuby is a basic (constitutively fluorescent) red fluorescent protein, monomeric engineered derivate of far-red fluorescent protein (RFP) isolated from members of the Entacmaea quadricolor. mRuby is a ~26 kDa protein that is optimally excited at a 558 nm and has a maximum of emission at 605 nm. This bright far-red fluorescent protein is used in research as a reporter to label and study the biology of the cell using whole body imaging.
Alternative names
far-Red antibody
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein (HIR78_01785 from Bacillus subtillis) and produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions
The antibody solution should be gently mixed before use.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:500
IHC (P) 1:50-1:500
IF 1:50-1:500
IEM 1:50-1:500

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ +++ +++ ND +++

Images

Anti-mRuby Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

320 €

Quantity / Unit Size

300 µg
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