Anti-TagGFP

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Catalog #: AB3477
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IHC (F), IHC (P), IF
Reactivity: Transfected cells
Description: Goat polyclonal antibody to TagGFP. TagGFP is a monomeric green fluorescent protein derived from the jellyfish Aequorea macrodactyla. It is an engineered GFP-like protein designed to provide high brightness and improved photostability. TagGFP has a molecular weight of approximately 27 kDa, with optimal excitation at ~482 nm and maximum emission at ~505 nm. It is widely used in molecular and cell biology as a fluorescent reporter to label proteins and study cellular processes such as protein localization, trafficking, and gene expression. The antibody recognizes TagGFP and TagGFP-tagged fusion proteins and can be used to detect their expression in various experimental systems, including Western blotting, immunofluorescence, and immunoprecipitation.
Alternative names: Green fluorescent antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence: N/A
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot.
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

Usage

Application	Values
WB	1:500-1:5,000
IHC (F)	1:50-1:1,000
IHC (P)	1:50-1:1,000
IF	1:50-1:1,000

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	IEM
Transfected cells	+++	+++	+++	+++	+++

Images

	Immunofluorescence – anti-TagGFP Ab using hCEC cells transfected with TagGFP-CAV1; cells were fixed with methanol and anti-TagGFP at 1/250;
	Anti-TagGFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;