Anti-BFP

(0) Reviews

(0) References

(0) Validations

(0) Q&A

Catalog #: AB3718
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IHC (F), IHC (P), IF, IEM
Reactivity: Transfected cells
Description: Goat polyclonal antibody to BFP (Blue fluorescent protein). BFP is a basic (constitutively fluorescent), monomeric engineered derivate of green fluorescent protein (GFP) isolated from Aequorea victoria. BFP is a ~27 kDa protein that is optimally excited at a 381 nm and has a maximum of emission at 445 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Alternative names: green fluorescent protein antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence: N/A
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize RFP (red fluorescent protein).
Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions: Avoid freeze/thaw cycles.

Usage

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA	IEM
Transfected cells	+++	+++	+++	+++	ND	+++

Images

	Anti-BFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-BFP Ab using NIH3T3 cells transduced with BFP-Rab5a; cells were fixed with methanol and anti-BFP at 1/100;
	Immunofluorescence – anti-BFP Ab using NIH3T3 cells transduced with BFP-Rab5a; cells were fixed with methanol and anti-BFP at 1/100;