Anti-mApple

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Catalog #: AB5421
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IHC (F), IHC (P), IF, IEM
Reactivity: Transfected cells
Description: Goat polyclonal antibody to mApple (Apple fluorescent protein). mApple is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mApple is a ~27 kDa protein that is optimally excited at a 568 nm and has a maximum of emission at 592 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.
Alternative names: red fluorescent protein, Apple antibody.
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence: N/A
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize GFP (green fluorescent protein).
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:500-1:5,000
IHC (F)	1:50-1:500
IHC (P)	1:50-1:500
IF	1:50-1:500
IEM	1:50-1:500

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA	IEM
Transfected cells	+++	+++	+++	+++	ND	+++

External References (1)

Kumar MS, Stallworth KM, Murthy AC et al. J Mol Biol 2023 Jan

PMID: 36690069

Images

	Immunofluorescence – anti-mApple Ab using hCEC cells transduced with mApple-Rab5a; cells were fixed with methanol and anti-mApple at 1/250;
	Anti-mApple Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-mApple Ab using hCEC cells transduced with mApple-Rab5a; cells were fixed with methanol and anti-mApple at 1/250;