mGarnet Polyclonal Antibody
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Catalog #
AB6430
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
None
Reactivity
Transfected cells
Description
Goat polyclonal antibody to mGarnet. mGarnet is a monomeric far-red fluorescent protein engineered from coral-derived fluorescent proteins, originating from the cnidarian Entacmaea quadricolor. It is designed as a bright and photostable reporter suitable for fusion tagging, live-cell imaging, and multicolor applications, particularly in the far-red spectrum. mGarnet is a basic (constitutively fluorescent) protein with improved maturation and stability compared to earlier variants. It typically exhibits a maximum excitation around ~598 nm and a maximum emission around ~670 nm. mGarnet-tagged proteins can be detected in Western blot at approximately 26–27 kDa plus the molecular weight of the fused protein. In immunofluorescence, antibody staining can enhance detection, particularly in fixed samples or low-expression systems. These antibodies are widely used in studies of protein localization, trafficking, and multiplex imaging, especially in combination with shorter-wavelength fluorophores for deep-tissue or low-background imaging applications.
Alternative names
mGarnet2, far-Red antibody
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration
3.00 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
Unconjugated
Immunogen
Affinity purified recombinant fluorescent protein produced in E. coli.
Antigen Sequence
N/A
Specificity
In lysates of transfected cells with the plasmid containing the fluorescent sequence, detects the recombinant protein by Western blot.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ ND ND ND ND ND

Images

Anti-mGarnet Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

375 €

Quantity / Unit Size

300 µg
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