Anti-GFP, DyLight®650
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Catalog #
AB66650
Category
Antibodies
Family
Fluorescent Proteins
Source
Goat
Application
WB, IHC (F), IF
Reactivity
Transfected cells
Description
Goat polyclonal antibody to GFP (green fluorescent protein) conjugated to DyLight® 650. GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Alternative names
Green fluorescent protein antibody.
Gene Identifier/Accession#
N/A
Form
Polyclonal antibody supplied as a 100 (2.5 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration
2.50 mg/ml
Isotype
IgG
Clonality
Polyclonal
Conjugation
DyLight®650
Immunogen
Purified recombinant peptide produced in E. coli.
Antigen Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Storage
Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
Special instructions
Avoid freeze/thaw cycles.

Usage

Application Values
WB 1:500-1:5,000
IHC (F) 1:50-1:1,000
IF 1:50-1:1,000

Reactivity Chart

Sample WB IHC (F) IHC (P) IF ELISA IEM
Transfected cells +++ +++ ND +++ ND ND

Images

Anti-GFP Ab conjugated to DyLight® 650 at 1/2,000 dilution using HEK293 transfected cell lysates at 30 µg per lane;

350 €

Quantity / Unit Size

250 µg
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