Anti-Rab27 Pan

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Catalog #: AB7223
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IHC (F), IHC (P), IF, ELISA
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to mouse Rab27. Rab27 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.
Alternative names: C25KG, GS2, GTP-binding protein Ram, rab-27, Rab27a, Rab27b, ras-related protein Rab-27A, ras-related protein Rab-27B, RAB27, RAM antibody.
Gene Identifier/Accession#: ENSG00000069974
Form: Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a and rab27b produced in E. coli.
Antigen Sequence: N/A
Specificity: Detects total Rab27 levels by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a and GFP-Rab27b cds.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Application	Values
WB	1:500-1:2,000
IHC (F)	1:50-1:400
IHC (P)	1:50-1:400
IF	1:50-1:250
ELISA	ND

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (3)

Schorb TS, PhD Thesis, University of Berlin, Germany, 2018
Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct.	PMID: 31613922
Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar	PMID: 35333565

Images

	Immunostaining of B6-RPE07 cells with Rab27 antibody (1/50);

	Immunostaining of primary RPE cells with Rab27 antibody (1/50);
	Anti-Rab27a antibody IHC staining of human mammary tissue; immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval; antibody concentration 1/200;
	Anti-Rab27 pan Ab at 1/500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunostaining of primary RPE cells with Rab27 antibody (1/50);