Anti-Rab1 Pan

(0) Reviews

(1) References

(0) Validations

(0) Q&A

Catalog #: AB7818
Category: Antibodies
Family: Vesicle Trafficking
Source: Goat
Application: WB, IHC (F), IHC (P), IF, ELISA
Reactivity: Human, Rat, Mouse, Monkey, Canine
Description: Goat polyclonal antibody to mouse Rab1. Rab1 belongs to the small GTPase superfamily, Rab family. Rab1 controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus.
Alternative names: RAB1A, RAB1B, RAS-associated protein RAB1A, ras-related protein Rab-1B, ras-related protein Rab-1A, ras-related protein Rab-1B, YPT1 antibody.
Gene Identifier/Accession#: ENSG00000138069
Form: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab1a and Rab1b produced in E. coli.
Antigen Sequence: N/A
Specificity: Detects total Rab1 protein by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab1a and GFP-Rab1b cds.
Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Special instructions: The antibody solution should be gently mixed before use.

Usage

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA
Human	+++	+++	+++	+++	ND
Rat	+++	+++	+++	+++	ND
Mouse	+++	+++	+++	+++	ND
Canine	+++	+++	+++	+++	ND
Monkey	+++	+++	+++	+++	ND

External References (1)

Mojallal M, Zheng Y, Hultin S et al. Nat Commun 2014 Aug 1;5:4557.

Images

	IHC of human appendix using anti-Rab1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1 Ab at 1:500/DAB detection.
	Immunofluorescence – anti-Rab1 Ab in COS-7 cells at 1/50 dilution; cells were fixed with 4% of PFA;
	IHC of mouse intestine using anti-Rab1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1 Ab at 1:500/DAB detection.
	Anti-Rab1 Ab at 1/1,000 dilution; 293HEK transfected lysate at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
	IHC of human pancreas using anti-Rab1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1 Ab at 1:250/DAB detection.