Anti-GFP

(0) Reviews

(0) References

(0) Validations

(0) Q&A

Catalog #: AB9066
Category: Antibodies
Family: Fluorescent Proteins
Source: Goat
Application: WB, IHC (F), IHC (P), IF, IEM
Reactivity: Transfected cells
Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Alternative names: Green fluorescent protein antibody
Gene Identifier/Accession#: N/A
Form: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in NaHCO3 100 mM pH 9. This antibody is epitope-affinity purified from goat antiserum and does not contain preservatives.
Concentration: 2.00 mg/ml
Isotype: IgG
Clonality: Polyclonal
Conjugation: Unconjugated
Immunogen: Purified recombinant peptide produced in E. coli.
Antigen Sequence: MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody does not recognize mCherry fluorescent protein.
Storage: Store at -20 C for long-term storage.
Special instructions: Avoid freeze/thaw cycles.

Usage

Reactivity Chart

Sample	WB	IHC (F)	IHC (P)	IF	ELISA	IEM
Transfected cells	+++	+++	+++	+++	ND	+++

Images

	Immunofluorescence – anti-GFP Ab using COS7 cells transduced with GFP; cells were fixed with methanol and anti-GFP at 1/250;
	Anti-GFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
	Immunofluorescence – anti-GFP Ab using COS7 cells transduced with GFP; cells were fixed with methanol and anti-GFP at 1/250;